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Assessment of the sanitary conditions of certified citrus nurseries production in Souss Massa Valley (Morocco)/ Ali El Aimani ; sup. M. Achouri e K. Djelouah

El Aimani, Ali

Tesi o dissertazioni 2005

Abstract

Biological, serological and molecular assays were applied and the basic material proved to be free from the virus and virus-like pathogens tested (CTV, CPsV, CVV, oak leaf pattern agent, CEVd, CCaVd, S. citri). Furthermore, the survey of 1054 citrus trees, carried out around the nurseries using DTBIA test, indicated an infection rate of 10% for CPsV and 2% for CVV, whereas CTV and S. citri were not reported. The inoculum density (ID) of Phytophthora spp. was determined in two rootstocks (Citrange Carrizo and sour orange) and in the nursery soil. In July, the ID was also estimated in different components of the soil mixture and in water. High values of ID and root infection (RI) were observed in July and the sour orange rootstock was more tolerant to Phytophthora root rot than Carrizo citrange. Only P. nicotianae was identified by molecular assay (PCR) thus demonstrating that it was the causal agent of Phytophthora root rot.
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