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Development of smart detection tools for some honey bee pathogens and preliminary survey on the sanitary status of honey bees in Morocco

Jabri, Badr-Eddine

Ressource électronique 2020

Résumé

In the last decade, the loss of the honey-bees population has been continuously increasing worldwide; this phenomenon is referred to as colony collapse disorder. Several causes are associated with this situation, including inter alia honey-bee infections by various pathogens. In the present study, protocols for rapid and sensitive diagnosis of some pathogens were set up and tested during a preliminary survey in three Moroccan regions. In this context, new primer pairs were designed and validated in qPCR for Nosema ceranae, Aspergillus flavus, Paenibacillus larvae and Black queen cell virus (BQCV). Preliminary monitoring of the main honey-bee pathogens in Moroccan apiaries was performed using the newly developed protocols and the already reported RT-PCR for the detection of Bee macula-like virus (BeeMLV), Deformed wing virus (DWV) and Slow bee paralysis virus (SBPV). Interestingly, N. [...]
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