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Improvement of Citrus exocortis viroid (CEVd) and Citrus cachexia viroid (CcaVd) detection

Abd-Elbacki, Ashraf Mousa Mahmoud

Tesi o dissertazioni - Istituto Agronomico Mediterraneo <Bari> - 2004

Abstract
Citrus exocortis viroid (CEVd) and Citrus cachexia viroid (CCaVd) detection was improved by setting up a new biological indexing method and comparing different molecular assays (molecular hybridisation, RT-PCR, sPAGE). Selected CEVd and CCaVd - infected sources, previously tested by sPAGE, were inoculated to Etrog citron cuttings, treated for rooting and kept in plastic bag at 35°C. Plants showed Ieaf epinasty 20 days after inoculation and reacted positively to dot-blot tests. This method proved to be reliable, time and space saving, easy and low cost. Comparing molecular assays in field diagnosis, RT-PCR showed to be the most sensitive method for CEVd and CCaVd detection from January to June, while dot-blot, tissue print and sPAGE were limited by the low viroid concentration in winter months. May can be considered the best month for starting viroid field detection, under Apulia conditions [...]
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M.Sc. 343

Document for internal consultation only

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6247
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M.Sc. 343a

Document for internal consultation only

Inventory
6248
Shelfmark
ARCH M.Sc. 343