Survey and molecular characterization of Pepino Mosaic virus (PepMV) infecting tomato crops in Morocco
Abstract
Pepino mosaic virus (PepMV) is currently causing great concern in the Moroccan greenhouse tomato industry, where it produces important economic losses. A survey was conducted from October 2015 to April 2016 on PepMV in seven different regions throughout Morocco. In a few plants, severe symptoms which could be associated to the virus were observed. A total of 315 samples of different varieties were collected and tested by DAS-ELISA for the presence of PepMV using a commercial kit. The obtained results showed that PepMV was widely distributed in the country with an infection rate of 21%, especially in the region of Souss Massa where 70% of the tested plants were infected. Representative samples from each region were selected and submitted for additional RT-PCR testing; 40% of the 83 tested samples were infected.
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Five isolates were selected for molecular characterization by sequencing the coat protein gene; all the isolates showed high nucleotide similarity with the Chilean CH2 strain (DQ000985), with a sequence homology ranging from 99.2% to 99.8%. These results should be considered for the development of control strategies for PepMV based on cross-protection using mild strains. Furthermore, the present study allowed the first report of Tomato torrado virus in the country.
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Biblioteca
Biblioteca CIHEAM Bari
Documento per sola consultazione interna
Collocazione
M.Sc. 786
Documento per sola consultazione interna
Collocazione
M.Sc. 786 A
Documento per sola consultazione interna
Collocazione
ARCH M.Sc. 786