Date palm fruit, one of the most nutritive and comprehensive fruit regarding health benefits is becoming an ideal substrate for deriving a range of value added products. The aim of this study was to develop the biotechnological protocol in order to exploit the functional properties of dates. Autochthonous lactic acid bacteria were isolated through culture-dependent methods and typed through Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis. Sixteen isolates representative of RAPD-PCR clusters were identified by partial 16S rRNA gene sequence and were screened based on the kinetics of growth on date palm juice used as model. Five autochthonous Lactobacillus plantarum strains were selected as the best performing strains.
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Nutritional and functional properties of fermented samples were compared with unfermented (raw) and chemically acidified one used as control, after freeze drying. Developed protocol enhanced nutritional and functional properties of date fruits. Total polyphenols, flavonoids content, antioxidant activities were significantly higher in fermented samples, compared to the controls. Our study reported, for the first time, genotypic diversity of the lactic acid bacteria microbiota associated with dates and provided the basis to exploit the potential of fermentation as novel bioprocessing in order to develop new functional food and supplements.
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