Dettaglio del documento
Investigation into Auchenorrhyncha species, putative vectors of "Bois noir" and "Flavescence dorée", in Apulian vineyards using different molecular techniques
Abstract
Flavescence dorée (FD) and Bois noir (BN) are the two most important phytoplasma diseases of grapevine in Italy. Their agents, Ca. Phytoplasma vitis and Ca. P. solani, are mainly vectored by Scaphoideus titanus (FD) and Hyalesthes obsoletus and other Auchenorrhyncha species (BN). To date, in Apulia region, only BN has been sporadically reported, but little is known about its putative vectors. In order to fill this gap, a total of 429 leafhoppers and froghoppers were captured from two different areas and identified and 190 of them were tested by nested-PCR and real-time PCR. Twenty-one individuals tested positive to phytoplasma infections, but none of them to BN or FD.
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Sequencing of 10 PCR-amplicons (1,250 bp) highlighted the presence of phytoplasmas of two different groups: elm yellow (16Sr V) and pigeon pea witches' broom (16Sr IX), probably acquired from infected wild plants in the vineyards. Eighty-two S. titanus individuals from a FD-infected vineyard in the region of Trentino (north Italy) were submitted to a comparative analysis by nested PCR, Real-time PCR and Real-Time LAMP. Ca. P. vitis was detected in 3 samples by RT-PCR, and only in 2 samples by the two other techniques.
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