Détection des principaux virus des agrumes dans le matériel végétal de multiplication Algérien et mise au point de la tecnique DTBIA pour l'identification du CVV
A survey was carried out in the main groves producing citrus propagating material in Algeria to detect psorosis (CPsV) and infectious variegation (CVV) viruses. The detection technique applied was DTBIA, using citrus flowers. The DTBIA protocol included the use of closed fresh flower ovaries, ovalbumin for the saturation of aspecific sites on the nitrocellulose membrane and a longer membrane incubation in the antibody and anti-mouse. The results highlighted a worrying situation in all tested groves given the broad spread of the two viruses. CPsV was widespread in the varietal collection and in the foundation block. As for CVV, its presence was more significant in the seed-plant block (9%). The biological and serological characterisation of four sources of Algerian psorosis showed the homogeneity of these strains and their similarity, to some extent, with other Mediterranean strains.
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The virus was isolated from Chenopodiaceae and purified, producing five distinct bands on a caesium sulphate gradient; only two bands reacted positively to ELISA test.
